Composite

Part:BBa_K1973023:Design

Designed by: Gabriel Ruiz Romero   Group: iGEM16_UPO-Sevilla   (2016-10-13)


miniTn7BB-Gm-nahR-Psal-pleD*


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4367
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4367
    Illegal BglII site found at 3051
    Illegal BglII site found at 3322
    Illegal BglII site found at 3608
    Illegal BglII site found at 4972
    Illegal BamHI site found at 5472
    Illegal BamHI site found at 6629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4367
    Illegal XbaI site found at 4382
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 4804
    Illegal NgoMIV site found at 5887
    Illegal NgoMIV site found at 6034
    Illegal AgeI site found at 6370
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1681
    Illegal BsaI site found at 5545
    Illegal SapI.rc site found at 2763


Design Notes

Designs are described in basic parts pages


Source

Basic parts were cloned into the miniTn7BB-Gm Backbone

References